Adipose Stem Cells from Buccal Fat Pad and Abdominal Adipose Tissue for Bone tissue Engineering

dc.contributor
Universitat Internacional de Catalunya. Departament d'Odontologia
dc.contributor.advisor
dc.contributor.author
Farré Guasch, Elisabet
dc.date.accessioned
2011-06-27T12:20:30Z
dc.date.available
2011-06-27T12:20:30Z
dc.date.issued
2011-05-20
dc.identifier.isbn
9789053353943
dc.identifier.uri
http://hdl.handle.net/10803/31987
dc.description.abstract
ABSTRACT Background and Objective: Stem cells offer an interesting tool for tissue engineering, but the clinical applications are limited by donor site morbidity and low cell number upon harvest. Recent studies have identified an abundant source of stem cells in subcutaneous adipose tissue. These adipose stem cells (ASC), are able to differentiate to several lineages and express multiple growth factors, which makes them suitable for clinical application. Buccal fat pad (BFP), an adipose encapsulated mass in the oral cavity, could represent an easy access source for dentists and oral surgeons. Biosynthetic substitutes such as β-tricalcium phosphate (β-TCP), hydroxyapatite (HA), and mixtures of HA/β-TCP (biphasic calcium phosphate; BCP) have been successfully used as bone graft biomaterials. Growth factors stimulating osteogenic differentiation are also interesting for bone tissue engineering applications. We aimed to investigate whether BFP is a rich source of ASC, and whether ASC triggered for only 15 min with bone morphogenetic protein-2 (BMP-2), and seeded onto different calcium phosphate scaffolds composed of β-TCP alone or mixtures of HA/β-TCP, could stimulate bone formation. Materials & Methods: ASC obtained from subcutaneous abdominal adipose tissue and BFP were counted and analyzed by flow cytometry, to determine ASC cell number, phenotype and percentage. At two weeks of culture, the multipotent differentiation potential of ASC from BFP was analyzed. Furthermore, fresh ASC either or not stimulated with 10ng/ml BMP-2 for 15min were seeded on different calcium phosphate scaffolds. ASC attachment, proliferation and osteogenic differentiation was analyzed and compared. Results: BFP contained ~30% of ASC. The ASC number obtained per gram of adipose tissue from BFP at one week of culture was 2-fold higher than in subcutaneous abdominal adipose tissue. Angiogenic marker expression was also higher, and ASC showed multipotent differentiation potential as well. Fifteen min BMP-2 treatment increased ASC cell proliferation and osteogenic differentiation on BCP composed of 60% HA and 40% β-TCP, but not on other scaffolds containing less percentage of HA. Conclusions: Buccal fat pad is a rich alternative source of ASC suitable for bone tissue engineering. Short stimulation of only 15 minutes with BMP-2 is enough to stimulate ASC proliferation and osteogenic differentiation. Therefore ASC could be treated shortly with BMP-2 and seeded on BCP with 60% HA to improve bone regeneration.
dc.format.extent
328 p.
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Universitat Internacional de Catalunya
dc.rights.license
ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.
dc.source
TDX (Tesis Doctorals en Xarxa)
dc.subject
Cèl•lules mare de teixit adipós (ASC)
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Células madre de tejido adiposo
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Adipose stem cells
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bola adiposa de Bichat (BFP)
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buccal fat pad
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enginyeria tisul•lar òssia
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bone tissue engineering
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proteïna morfogenètica òssia-2 (BMP-2)
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bone morphogenetic protein-2
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biomaterials
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scaffolds
dc.subject.other
Medicina, Odontologia
dc.title
Adipose Stem Cells from Buccal Fat Pad and Abdominal Adipose Tissue for Bone tissue Engineering
dc.type
info:eu-repo/semantics/doctoralThesis
dc.type
info:eu-repo/semantics/publishedVersion
dc.subject.udc
61
dc.subject.udc
616.3
dc.contributor.director
Casals Farré, Núria
dc.contributor.codirector
Martí Pagès, Carles
dc.contributor.tutor
Klein-Nulend, Jenneke
dc.rights.accessLevel
info:eu-repo/semantics/openAccess
dc.identifier.dl
B.25086-2011


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