Ultrashort laser pulse measurement for multiphoton microscopy

dc.contributor
Universitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions
dc.contributor.author
Amat Roldan, Ivan
dc.date.accessioned
2014-01-17T12:00:06Z
dc.date.available
2014-01-17T12:00:06Z
dc.date.issued
2013-06-21
dc.identifier.uri
http://hdl.handle.net/10803/128800
dc.description.abstract
In this thesis, we address specific efforts towards developing the precise aspect of ultrashort laser pulse measurement in the context of biomedical research. The motivation for pursuing these new developments was triggered by the vision of developing fundamental tools that will enable to control matter by means of light with exquisite precision with the added difficulty of being next to biological samples which are extremely sensitive and fragile. For this, light matter interaction needs to be extremely well controlled to avoid undesired effects, like cell damage due to the high peak intensity values of ultrashort laser pulses, as well as promoting specific physical processes like two-photon fluorescence excitation of a desired fluorophore embedded in some biochemical environment. We focus in the two major bottlenecks regarding ultrashort laser pulse measurements for multiphoton microscopy, that aim for developing (1) new techniques for full characterization of ultrashort pulses under different experimental conditions and (2) new material with specific nonlinear properties that enable to obtain ultrashort pulse measurements that properly catch the temporal shape of light and at the same time can be readily found in biomedical lab, specially cost effective, non fragile and non-toxic. Combination of these two complementary strategies provides a new ground where it is possible to characterise an ultrashort pulse at the sample plane of a multiphoton microscope in a regular biomedical research facility. Importantly, we approach ultrashort pulse characterisation by developing a different theoretical framework to the state-of-the-art and we propose few initial experiments that preliminary support our theoretical statements in the form of new optical techniques. These findings are then experimentally tested under different conditions, such different optical setups and different pulsed regimes in order to evaluate the feasibility of the tools to measure ultrashort pulses in conditions that were prohibitive at the time this thesis was started. The scope of this thesis outlines the potential of such techniques, but further efforts shall be addressed to assess feasibility, robustness and further limitations.
dc.format.extent
225 p.
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Universitat Politècnica de Catalunya
dc.rights.license
ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.
dc.source
TDX (Tesis Doctorals en Xarxa)
dc.title
Ultrashort laser pulse measurement for multiphoton microscopy
dc.type
info:eu-repo/semantics/doctoralThesis
dc.type
info:eu-repo/semantics/publishedVersion
dc.subject.udc
535
dc.contributor.director
Loza Álvarez, Pablo
dc.embargo.terms
cap
dc.rights.accessLevel
info:eu-repo/semantics/openAccess
dc.identifier.dl
B. 3850-2014


Documentos

TIAR1de1.pdf

13.44Mb PDF

Este ítem aparece en la(s) siguiente(s) colección(ones)