Momentum measurements of single-beam traps and quantitative holographic experiments: two sides of the same coin

Author

Farré Flaquer, Arnau

Director

Montes Usategui, Mario

Martín Badosa, Estela

Date of defense

2012-07-03

Legal Deposit

B. 24017-2012

Pages

144 p.



Department/Institute

Universitat de Barcelona. Departament de Física Aplicada i Òptica

Abstract

After an intense development of optical tweezers as a biophysical tool during the last decades, quantitative experiments in living cells have not found in this technique its best ally, due, in part, to the lack of a reliable method to measure forces in complex environments. The attempts to overcome this problem either require complicated in situ calibrations, which make their use impossible in the study of dynamic processes, or they are inaccurate. Using a different approach, Steven Smith at Carlos Bustamante’s lab at the University of Berkeley developed a method based on the direct measurement of the momentum change of the trapping beam. However, its diffusion has been modest mainly because it requires a counter-propagating optical trapping system, which is difficult to implement and combine with other techniques. Although it has not been used for this purpose yet, it seems a more suitable method for in vivo experiments since the measurement depends only on some properties of the sensor apparatus but not on the experiment itself. On the other hand, the use of holographic optical tweezers in molecular biology experiments involving force and position measurements is still far from established. The existence of different effects associated to the use of spatial light modulators to create the optical traps has restricted their use. In this thesis, I present the work that I carried out in the Optical Trapping Lab – Grup de Biofotònica at the University of Barcelona related to these two subjects. During these years, I have focused on the implementation of the force detection method based on the conservation of the light momentum in single-beam optical traps, and on the analysis of several aspects of holographic tweezers oriented to their use in quantitative experiments.

Keywords

Òptica; Óptica; Optics; Làsers; Láseres; Lasers; Pinces òptiques; Pinzas ópticas; Optical tweezers; Microscòpia; Microscopía; Microscopy

Subjects

535 - Optics

Knowledge Area

Ciències Experimentals i Matemàtiques

Documents

AFF_PhD_THESIS.pdf

17.09Mb

 

Rights

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