Optimització del diagnòstic microbiològic de la bacterièmia en un hospital sense atenció continuada. Aplicació d’una PCR Multiplex sobre frasc d’hemocultiu
llistat de metadades
Alternative title
Optimization of microbiological diagnosis of Bacteraemia in a hospital without 24 hour-a-day service. Application of a multiplex PCR on blood culture
Author
Director
Moreno Muñoz, Rosario
Tutor
Maciá Soler, Maria Loreto
Date of defense
2022-06-22
Pages
131 p.
Department/Institute
Universitat Jaume I. Escola de Doctorat
Doctorate programs
Programa de Doctorat en Ciències de la Infermeria
Abstract
The microbiological diagnosis of bacteremia is essential to an early establishment of the approppriate treatment. We present a study of 133 patients performing multiplex PCR (FA-BCID) in parallel with conventional blood culture. The diagnosis was advanced by about two days on positive blood culture. The concordance was 79%, 95% CI (66.86), due to the detection of S.pneumoniae which was not isolated. In 22% of patients, the ATB was modified when the PCR result was known. The percentage of polymorphonuclear cells and the age are the only relevant parameters in order to condition a positive PCR result. Experimental PCR was performed in non-positivized blood cultures. In six cases the identification could be considerably advanced. The concordance when PCR was negative with the culture was 82.3%. Especially in hospitals without continuous care, FA-BCID is a good tool that can speed up the diagnosis but cannot replace traditional blood culture.
Keywords
Diagnòstic; Bacterièmia; Sèpsia; Molecular; Microbiologia; Atenció continuada; Diagnosis; Bacteraemia; Sepsis; Microbiology
Subjects
614 - Public health and hygiene. Pollution. Accident prevention