2024-03-28T21:39:18Zhttps://www.tdx.cat/oai/requestoai:www.tdx.cat:10803/71252024-03-15T10:57:24Zcom_10803_236col_10803_690278
nam a 5i 4500
Retención intrónica
Forkhead
Fkh2
Mei4
Rem1
Inmunoprecipitación de cromatina
Recombinación
Levadura de fisión
Promotor
Ciclina
Meiosis
Schizosaccharomyces pombe
Procesamiento
Intron retention
Forkhead
Fkh2
Mei4
Rem1
Chromatin Immunoprecipitation
Promoter
Fission yeast
Recombination
Cyclin
Meiosis
Schizosaccharomyces pombe
Splicing
Promoter-driven splicing regulation in fission yeast
[Barcelona] :
Universitat Pompeu Fabra,
2011
Accés lliure
http://hdl.handle.net/10803/7125
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AAMMDDs2011 sp ||||fsm||||0|| 0 eng|c
9788469234785
Moldón Vara, Alberto,
autor
Programa de doctorat en Biomedicina,
degree
Tesi
Doctorat
Universitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut
2008
Universitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut
Tesis i dissertacions electròniques
Ayté del Olmo, José,
supervisor acadèmic
TDX
The meiotic cell cycle is modified from the mitotic cell cycle by having a premeiotic S phase which leads to high levels of recombination, two rounds of nuclear division with no intervening DNA synthesis, and a reductional pattern of chromosome segregation. Rem1 is a cyclin that is expressed only during meiosis in the fission yeast Schizosaccharomyces pombe. Cells in which rem1 has been deleted show a decreased intragenic meiotic recombination and a delay at the onset of meiosis I. When ectopically expressed in mitotically growing cells, Rem1 induces a G1 arrest followed by severe mitotic catastrophes. Here we show that rem1 expression is regulated at the level of both transcription and splicing, encoding for two proteins with different function depending on the intron retention. We have determined that the regulation of rem1 splicing is not dependent on any transcribed region of the gene. Furthermore, when the rem1 promoter is fused to other intron-containing genes, the chimeras show a meiotic-specific regulation of splicing, exactly as endogenous rem1. This regulation is dependent on two transcription factors of the forkhead family, Mei4 and Fkh2. While Mei4 induces both transcription and splicing of rem1, Fkh2 is responsible for the intron retention of the transcript during vegetative growth and pre-meiotic S phase.
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