2024-03-28T23:18:26Zhttps://www.tdx.cat/oai/requestoai:www.tdx.cat:10803/6670302020-05-13T02:00:20Zcom_10803_1col_10803_400197
nam a 5i 4500
Arabidopsis thaliana
Hibridació vegetal
Hibridación vegetal
Plant hybridization
RNA
ARN
Pseudomonas
Genetics of natural variation and environmental modulation of immune-related hybrid incompatibilities in Arabidopsis
[Barcelona] :
Universitat de Barcelona,
2019
Accés lliure
http://hdl.handle.net/10803/667030
cr |||||||||||
AAMMDDs2019 sp ||||fsm||||0|| 0 eng|c
Atanasov, Kostadin Evgeniev,
autor
1 recurs en línia (204 pàgines)
Programa de Doctorat en Biotecnologia
Tesi
Doctorat
Universitat de Barcelona. Facultat de Farmàcia i Ciències de l'Alimentació
2019
Universitat de Barcelona. Facultat de Farmàcia i Ciències de l'Alimentació
Tesis i dissertacions electròniques
Alcázar Hernández, Rubén,
supervisor acadèmic
Fernández Tiburcio, Antonio,
supervisor acadèmic
Alcázar Hernández, Rubén,
supervisor acadèmic
TDX
Arabidopsis thaliana is a small ruderal weed with prominent north-hemisphere distribution. It has an autogamous style of reproduction, whereas about 3% of the natural population can exhibit outcrosses. Some of these outcrosses produce incompatible hybrids exhibiting temperature-dependent autoimmunity. The hybridization between accessions Ler (from North-Europe) and Kas-2 (from Central Asia) produces an incompatible hybrid. The genetics underlying the incompatibility is caused by the deleterious epistasic interactions between a unique Ler haplotype of RPP1-like genes and the SRF3 Kas-2 receptor-like kinase. The incompatibility exhibits recessive mendelian inheritance pattern and hybrids display strong dwarfism, spontaneous cell death, and sterility at 14 ◦C – 16 ◦C. By using EMS mutagenesis and CRISPR/Cas-9 gene editing on Ler/Kas-2 NIL plants, 15 RPP1-like intragenic loss-of-function mutations were identified. Furthermore, the impact of suppression of incompatibility was investigated in terms of disease resistance against hemibiotrophic or biotrophic pathogens. Moreover, changes on the global transcriptome and metabolite profiling were studied. The contribution of individual RPP1-like genes for the recognition of a local Hpa isolate (Gw) was carried out by cloning ATR1 effector and performing transient expression assays in tobacco plants. The environmental modulation of autoimmunity was studied with a focus on the nitrogen-based mineral nutrition. We found that increasing ammonium was sufficient to suppress the incompatibility and the mechanism behind implies signaling through nitric oxide and the contribution of the systemic acquired response master gene, NPR1. Furthermore, we suggest that ammonium enriched media has an impact on resistance to Pst turning the enhanced resistance on incompatible hybrids down to the parental lines. Finally, we investigated the natural variation to the plant herbicide, guazatine, on a set of 107 accessions. We found an association for the chlorophyllase 1 gene which was selected for functional validation together with the paralog chlorophyllase 2 gene. Chlorophyllase individual mutants and double mutants exhibit increased tolerance to guazatine. No geographical pattern between the tolerant genotype and gene phylogeny was found, whereas high proportion of tolerant accessions was detected in populations for Germany.
b
ES-BaCBU
cat
rda
ES-BaCBU
text
txt
rdacontent
informàtic
c
rdamedia
recurs en línia
cr
rdacarrier