2024-03-28T12:18:08Zhttps://www.tdx.cat/oai/requestoai:www.tdx.cat:10803/4016472017-09-03T13:19:59Zcom_10803_1col_10803_400197
nam a 5i 4500
Plantes medicinals
Plantas medicinales
Medicinal plants
Biotecnologia vegetal
Biotecnología vegetal
Plant biotechnology
New biotechnological systems for the research on aryltetralin lignans in Linum album
[Barcelona] :
Universitat de Barcelona,
2017
Accés lliure
http://hdl.handle.net/10803/401647
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Lalaleo Córdova, Liliana Paulina,
autor
1 recurs en línia (194 pàgines)
Tesi
Doctorat
Universitat de Barcelona. Facultat de Farmàcia i Ciències de l'Alimentació
2017
Universitat de Barcelona. Facultat de Farmàcia i Ciències de l'Alimentació
Tesis i dissertacions electròniques
Bonfill Baldrich, Ma. Mercedes,
supervisor acadèmic
Alcázar Hernández, Rubén,
supervisor acadèmic
Cusidó Vidal, Rosa M.,
supervisor acadèmic
TDX
Linum album is an herbaceous plant with medical interest due to its content of podophyllotoxin (PTOX), an aryltetralin lignan with cytotoxic activity. The study of biotechnological alternatives has generated big interest. Organ and cell in vitro cultures offer the possibility of suppling aryltetralin lignans in accordance with a sustainable and rational utilization of biodiversity, however they need to be improved. Thus, in this work we analyzed the lignan accumulation patterns in four biotechnological systems of L.album in order to find alternatives of bioproduction. The four biotechnological platforms were established with wild type and transformed cell suspension cultures, adventitious roots isolated from in vitro plants, and hairy roots. The predominant lignan produced by wild type and transformed cells was PTOX. The main lignan produced by adventitious and hairy roots was 6- MPTOX, adventitious roots showed to be more productive, and we can infer that the transformation did not change the lignan patterns. In addition, we have studied the response to the elicitor coronatine in these systems. Transformed cells were the most sensitive after elicitation and showed an arrest of biomass growth in relation to other systems. In general, the elicitation increased the lignan content, but the more elicited route in each system resulted to be the less productive. Transcript profiling changed in elicited conditions, specially for the PLR gene in transformed cells. The analysis of morphogenesis development in the production of podophyllotoxin derivatives in callus cultures of Linum album showed that, the absence of plant growth regulators are a predominant factor, to induce the organogenic
and the bioproduction as well. Finally, approaches aiming at increasing podophyllotoxin content require multiple enzymatic steps that facilitate the metabolic flow to the final products, most of them forming multiprotein complexes. Based on this, the Yeast two hibrid system allowed to identify CTB5 as a potential interactor of the PLR protein of Linum album, and evidences in other species indicate that CTB5 participates in the phenylpropanoid pathway.
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