2024-03-28T08:56:45Zhttps://www.tdx.cat/oai/requestoai:www.tdx.cat:10803/71572024-03-15T10:57:33Zcom_10803_236col_10803_690278
2009-07-13
urn:hdl:10803/7157
Analysis of mouse kreisler mutants reveals new roles of hindbrain-derived signals in the establishment of the otic neurogenic domain
Vázquez Echeverría, Citlali
citlali.vazquez@upf.edu
true
Pujades, Cristina
hindbrain
inner ear
patterning
labyrinth (ear) - physiology
kreisler/MafB
rhombencephalon
mice as laboratory animals - embriology
developmental neurobiology
laberint (orella) - fisiologia
romboencèfal
ratolins (animals de laboratori) - embriologia
neurobiologia del desenvolupament
FGF
Krox20
neurogenesis
The inner ear, the sensory organ responsible for hearing and balance, contains specialized sensory and non-sensory epithelia arranged in a highly complex threedimensional structure. To achieve this complexity, a tight coordination between morphogenesis and cell fate specification is essential during otic development. Tisúes surrounding the otic primordium, and more particularly the adjacent segmented hindbrain, have been implicated in specifying structures along the anteroposterior and dorsoventral axes of the inner ear. In this work we have first characterized the generation and axial specification of the otic neurogenic domain, and second, we have investigated the effects of the mutation of kreisler/MafB -a gene transiently expressed in the rhombomeres 5 and 6 of the developing hindbrain- in early otic patterning and cell specification. We show that kr/kr embryos display an expansion of the otic neurogenic domain, due to defects in otic patterning. Although many reports have pointed to the role of FGF3 in otic regionalization, we provide evidence that FGF3 is not sufficient to govern this process. Neither Krox20 nor Fgf3 null mutant embryos, in which Fgf3 is either downregulated or absent in r5 and r6, present ectopic otic neuroblasts in the otic primordium. However, Fgf3-/-Fgf10-/- double mutants show a phenotype very similar to kr/kr embryos: they present ectopic neuroblasts along the AP and DV otic axes. Finally, and remarkably, partial rescue of the kr/kr phenotype is obtained when Fgf3 or Fgf10 are ectopically expressed in the hindbrain of kr/kr embryos. These results highlight a compensatory mechanism between FGFs, and the importance of hindbrain-derived signals in instructing otic patterning and the establishment of the neurogenic domain.
2011-04-12T16:28:51Z
2009-07-13
2008-12-18
2009-07-13
info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
9788469248621
http://www.tdx.cat/TDX-0713109-133451
http://hdl.handle.net/10803/7157
B.32292-2009
eng
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info:eu-repo/semantics/openAccess
Universitat Pompeu Fabra
TDX (Tesis Doctorals en Xarxa)